File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig Miller\Results\Saliva IgA Luitpold d57 070113.rbx
Analyte: IgA (63)
Acquisition Date: 01-Jul-2013, 01:00 PM
Reader Serial Number: LX10008241401
Plate ID: 
RP1 PMT (Volts): 647.13
RP1 Target: 17026
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1,B1,C1,D1 0 69.8 69.8 3.86 1.93 5.54
IgA (63) S1 E1,F1 2 Standard 1 12343 12273.3 0 0 0 520.38 500 104 520.38
IgA (63) S2 G1,H1 0 Standard 2 9550.5 9480.8 109.6 77.5 1.15 232.68 250 93 232.68
IgA (63) S3 A2,B2 0 Standard 3 7474 7404.3 156.98 111 2.1 130.66 125 105 130.66
IgA (63) S4 C2,D2 0 Standard 4 5010 4940.3 258.8 183 5.17 62.61 62.5 100 62.61
IgA (63) S5 E2,F2 0 Standard 5 3115 3045.3 72.12 51 2.32 31.18 31.25 100 31.18
IgA (63) S6 G2,H2 0 Standard 6 1773.3 1703.5 32.17 22.75 1.81 15.45 15.63 99 15.45
IgA (63) S7 A3,B3 0 Standard 7 991.3 921.5 60.46 42.75 6.1 7.89 7.81 101 7.89
IgA (63) S8 C3,D3 2 Standard 8 557.5 487.8 0 0 0 3.89 3.91 100 3.89
IgA (63) X1 E3,F3 0 4661 3472.5 3402.8 126.57 89.5 3.64 36160.93 36160.93
IgA (63) X2 G3,H3 0 4331 482.5 412.8 3.54 2.5 0.73 *3170.62 ***
IgA (63) X3 A4,B4 0 4326 1286 1216.3 16.97 12 1.32 10647.35 10647.35
IgA (63) X4 C4,D4 0 4651 4826 4756.3 223.45 158 4.63 58941.83 58941.83
IgA (63) X5 E4,F4 0 4653 2850.8 2781 37.12 26.25 1.3 27739.96 27739.96
IgA (63) X6 G4,H4 0 4654 2130.5 2060.8 81.32 57.5 3.82 19244.87 19244.87
IgA (63) X7 A5,B5 0 4655 3037 2967.3 48.08 34 1.58 30147.77 30147.77
IgA (63) X8 C5,D5 0 4657 1885.8 1816 66.11 46.75 3.51 16620.58 16620.58
IgA (63) X9 E5,F5 0 4658 1514.5 1444.8 10.61 7.5 0.7 12854.71 12854.71
IgA (63) X10 G5,H5 0 4659 2919 2849.3 35.36 25 1.21 28611.45 28611.45
IgA (63) X11 A6,B6 0 4662 1138.5 1068.8 65.76 46.5 5.78 9258.71 9258.71
IgA (63) X12 C6,D6 0 4663 1362 1292.3 89.1 63 6.54 11373.32 11373.32
IgA (63) X13 E6,F6 0 4665 302.5 232.8 3.54 2.5 1.17 *1213.98 ***
IgA (63) X14 G6,H6 0 4666 1109.3 1039.5 8.13 5.75 0.73 8986.14 8986.14
IgA (63) X15 A7,B7 0 4667 1803.8 1734 16.62 11.75 0.92 15767.62 15767.62
IgA (63) X16 C7,D7 0 4669 1137.3 1067.5 25.1 17.75 2.21 9247.04 9247.04
IgA (63) X17 E7,F7 0 4670 2261 2191.3 1.41 1 0.06 20695.03 20695.03
IgA (63) X18 G7,H7 0 4671 2157.5 2087.8 19.09 13.5 0.88 19541.91 19541.91
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1 0 68 68 100
IgA (63) B B1 0 70 70 100
IgA (63) B C1 0 66 66 129
IgA (63) B D1 0 75 75 107
IgA (63) S1 E1 0 12343 12273.3 520.38 500 104 520.38 109
IgA (63) S1 F1 1 12468 12398.3 500 141
IgA (63) S2 G1 0 9473 9403.3 227.73 250 91 227.73 111
IgA (63) S2 H1 0 9628 9558.3 237.75 250 95 237.75 100
IgA (63) S3 A2 0 7585 7515.3 134.8 125 108 134.8 117
IgA (63) S3 B2 0 7363 7293.3 126.63 125 101 126.63 115
IgA (63) S4 C2 0 5193 5123.3 66.41 62.5 106 66.41 165
IgA (63) S4 D2 0 4827 4757.3 58.96 62.5 94 58.96 131
IgA (63) S5 E2 0 3166 3096.3 31.87 31.25 102 31.87 177
IgA (63) S5 F2 0 3064 2994.3 30.5 31.25 98 30.5 100
IgA (63) S6 G2 0 1750.5 1680.8 15.22 15.63 97 15.22 86
IgA (63) S6 H2 0 1796 1726.3 15.69 15.63 100 15.69 131
IgA (63) S7 A3 0 1034 964.3 8.29 7.81 106 8.29 100
IgA (63) S7 B3 0 948.5 878.8 7.5 7.81 96 7.5 100
IgA (63) S8 C3 1 549 479.3 3.91 105
IgA (63) S8 D3 0 557.5 487.8 3.89 3.91 100 3.89 110
IgA (63) X1 E3 0 3562 3492.3 37467.5 37467.5 149
IgA (63) X1 F3 0 3383 3313.3 34879.23 34879.23 109
IgA (63) X2 G3 0 480 410.3 *3146.06 *** 105
IgA (63) X2 H3 0 485 415.3 *3195.14 *** 127
IgA (63) X3 A4 0 1274 1204.3 10533.41 10533.41 111
IgA (63) X3 B4 0 1298 1228.3 10761.47 10761.47 115
IgA (63) X4 C4 0 4668 4598.3 55915.22 55915.22 131
IgA (63) X4 D4 0 4984 4914.3 62078.96 62078.96 153
IgA (63) X5 E4 0 2824.5 2754.8 27408.05 27408.05 120
IgA (63) X5 F4 0 2877 2807.3 28073.69 28073.69 105
IgA (63) X6 G4 0 2188 2118.3 19879.31 19879.31 110
IgA (63) X6 H4 0 2073 2003.3 18617.41 18617.41 128
IgA (63) X7 A5 0 3003 2933.3 29701.21 29701.21 113
IgA (63) X7 B5 0 3071 3001.3 30597.53 30597.53 115
IgA (63) X8 C5 0 1932.5 1862.8 17112.62 17112.62 108
IgA (63) X8 D5 0 1839 1769.3 16132.74 16132.74 125
IgA (63) X9 E5 0 1507 1437.3 12781.03 12781.03 100
IgA (63) X9 F5 0 1522 1452.3 12928.47 12928.47 100
IgA (63) X10 G5 0 2944 2874.3 28933.79 28933.79 121
IgA (63) X10 H5 0 2894 2824.3 28290.79 28290.79 100
IgA (63) X11 A6 0 1185 1115.3 9693.82 9693.82 127
IgA (63) X11 B6 0 1092 1022.3 8825.78 8825.78 102
IgA (63) X12 C6 0 1299 1229.3 10770.99 10770.99 121
IgA (63) X12 D6 0 1425 1355.3 11981.14 11981.14 105
IgA (63) X13 E6 0 300 230.3 *1181.05 *** 100
IgA (63) X13 F6 0 305 235.3 *1246.56 *** 121
IgA (63) X14 G6 0 1103.5 1033.8 8932.66 8932.66 110
IgA (63) X14 H6 0 1115 1045.3 9039.66 9039.66 126
IgA (63) X15 A7 0 1815.5 1745.8 15889.07 15889.07 128
IgA (63) X15 B7 0 1792 1722.3 15646.42 15646.42 103
IgA (63) X16 C7 0 1119.5 1049.8 9081.56 9081.56 100
IgA (63) X16 D7 0 1155 1085.3 9412.84 9412.84 101
IgA (63) X17 E7 0 2262 2192.3 20706.29 20706.29 119
IgA (63) X17 F7 0 2260 2190.3 20683.78 20683.78 101
IgA (63) X18 G7 0 2144 2074.3 19393.2 19393.2 133
IgA (63) X18 H7 0 2171 2101.3 19691 19691 127
Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature
***Value not available / --- = Designated as an outlier
*Value extrapolated beyond standard range
OOR = Out of Range / OOR> = Out of Range Above  / OOR< = Out of Range Below
Exp Conc = Expected Concentration  /  Obs Conc = Observed  Concentration
Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130%
Regression Type: Logistic - 5PL
Std. Curve: FI = 170.782 + (20938 - 170.782) / ((1 + (Conc / 20.949)^-0.542293))^3.34556
FitProb. = 0.8612, ResVar. = 0.2503